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Sampling Plan: Fry will be collected 17 days after stocking the brood pond with adults. The fry will be graded for size by passing them through fine mesh such that only fish between 8 and 12 mm length will be used in the experiment. The exact nature of the treatments depends on the outcome of Study 1; . if Study 1 demonstrates that two immersions are necessary for masculinization, then the study will focus on determining if immersions on any two days between collection of the fry (Day 1) and Day 4 results in masculinization. The treatment groups will be:

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