Liquitech steroids

To detect SRC-1 protein expression, bone cell lysates were separated by SDS-PAGE and transferred onto nitrocellulose membranes. Membranes were probed with a goat polyclonal antibody raised against a carboxyl terminus peptide of human SRC-1 that is identical to the corresponding mouse sequence (1:1000 dilution, C-20; Santa Cruz Biotechnology) and a rabbit polyclonal antibody raised against a recombinant protein corresponding to amino acids 350-690 mapping within an internal region of SRC-1 of mouse origin (1:1000 dilution, M-341; Santa Cruz Biotechnology) and then a peroxidase-conjugated second antibody. Blots were visualized using an ECL detection kit (Amersham Biosciences).

Liquitech steroids

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